Thermo sirna设计
Webb本实验采用HO-1小干扰RNA(small interfering RNA,siRNA)技术初步探讨HO-1对LPS诱导培养的大鼠肝细胞内质网应激的影响。 1.1 试剂与仪器 大鼠正常肝细胞系BRL购自中国科学院上海细胞研究所细胞库。 WebbHigh quality siRNA reagents for potent gene silencing. RNA interference (RNAi) using small, interfering RNA (siRNA) is the best way to effectively knock down gene expression …
Thermo sirna设计
Did you know?
WebbThe Silencer Select Human Membrane Trafficking siRNA Library contains 0.25 nmol of each highly potent siRNA, sufficient for at least 500 transfections (at 5 nM siRNA, 100 µL transfection volume). Flexibility to meet your workflow needs. Thermo Fisher Scientific offers a comprehensive list of pre-defined or custom siRNA libraries to human ... WebbsiRNA Small interfering RNA/silencing RNA (siRNA) is a class of double-stranded, non-coding RNA molecule, 20-25 base pairs in length. As part of the RNA interference pathway, it regulates gene expression by degrading mRNA after transcription, preventing translation.
WebbSilencer ™ Select siRNA是第二代Ambion™ siRNA产品。 这些siRNA集成了最新改良的siRNA设计,脱靶效应预测算法和化学修饰,能够为用户生成具有卓越的效力、强度与 … Webb胰腺癌细胞株mia paca-2和sw1990购自美国atcc细胞库,胎牛血清(fbs)、转染用opti-mem培养基购自美国gibco公司,rt试剂盒、pcr相关sybrp、逆转录试剂盒购自鼎国生物有限公司,cck-8试剂盒购自日本同仁化学研究所,细胞周期试剂盒购自美国thermo公司,兔抗人单克隆抗体细胞周期蛋白d1(cyclind1)、细胞周期蛋白 ...
WebbStealth RNAi siRNA 或 siRNA 双链的转染浓度通过将所用摩尔量除以取决于最终转染体积(即起始培养基体积加转染混合物体积)确定。 使用强效 siRNA 时,我们通常通过针 …
Webb10 sep. 2024 · siGENOME™ siRNA产品信息: Dharmacon产品的性能保证中的标准条件适用于:以100nM终浓度的siRNA转染细胞24h-48h在mRNA水平检测靶基因的转录;同时必须使用Dharmacon提供的阳性对照双链siRNA或者已经确认的有功能的siRNA双链,以确保实验中获得最佳的转染效率;较低的转染效率必将导致沉默水平的下降;同时不同细胞 …
WebbRosetta siRNA设计算法利用位点特异性打分矩阵和种子区域知识,预测目标基因最具效率和特异性的序列。 该算法的规则基于超过三年的基因沉默实验实证数据开发。 产品优势 同类产品之中的佼佼者,基因沉默效果有 保障 有效敲低低丰度信使 使用 11 阳性对照 siRNA 简化转染优化 使用 8 阴性对照 siRNA 区分序列特异性沉默和非特异性效应 数百种经过功 … septic honey dipperWebb14 nov. 2015 · Small interference RNA (siRNA) transfection fourpre-designed siRNAs targeted humanCETP mRNA (Cat. L-009485-00)were obtained from Dharmacon (Lafayette, CO). silencernegative control siRNA obtainedfrom Applied Biosystem. cellswere mixed 50nM siRNA using siPORT NeoFX siRNA transfection reagent (Ambion) 6-wellplates. septic hookupWebb7 apr. 2008 · Artificially synthesized short interfering RNAs (siRNAs) are widely used in functional genomics to knock down specific target genes. One ongoing challenge is to guarantee that the siRNA does not elicit off-target effects. theta googleマップWebbUse our GeneAssist™ Custom siRNA Builder to design and order a custom siRNA. Silencer™ Validated siRNA Invitrogen™ Silencer® Select siRNAs are classic 21-mers … septic holding tanks for small cabinsWebb23 mars 2013 · Step 1: Enter an accession number or provide a nucleotide sequence. Accession number: OR. Nucleotide sequence: Enter only A, C, G, T, and U. See the online … the tag outletWebb如果您希望设计自己的siRNA,可根据以下指导准则从不同物种中选择siRNA靶向位点。 然后,通过化学合成、体外转录、载体或PCR产物表达等方式进行制备相应的siRNA。 1. … septic hymanWebbThe siDESIGN Center is an advanced, user-friendly siRNA design tool, which significantly improves the likelihood of identifying functional siRNA. One-of-a-kind options are … theta good investment