Incipient cytorrhysis assay
WebJan 15, 2003 · Incipient cytorrhysis of appressoria was assessed by determining the percentage of appressoria that collapsed after exposure to a range of glycerol … WebJan 26, 2024 · To investigate whether the reduced pathogenicity of ΔMopcs60 was associated with appressorium-mediated penetration, we first determined the rate of …
Incipient cytorrhysis assay
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Webincipient cytorrhysis assays indicate that turgor pressure begins to be generated in the appressorium from 16 h (Fig. 1) and penetration of the rice cuticle can occur from 24 h … WebAug 3, 2024 · Glycogen staining and cytorrhysis assays Appressorium turgor was measured using an incipient cytorrhysis assay. Twenty microlitres of the conidial suspension (5 × 10 4 spores/ml) was placed on hydrophobic coverslips and incubated in a humid chamber for 24 h at room temperature.
WebApr 6, 2007 · Appressorial turgor was estimated using incipient cytorrhysis (cell collapse) assays ( 6 ). Conidia in 20-μl droplets (1 × 10 6 conidia/ml) were induced to form appressoria on plastic microscope coverslips incubated in a humid chamber for 24 h or 48 h. WebApr 1, 2002 · CYP1 putatively encodes a mitochondrial and cytosolic form of cyclophilin, and targeted gene replacement has shown that CYP1 acts as a virulence determinant in rice blast. Cyp1 mutants show reduced virulence and are impaired in associated functions, such as penetration peg formation and appressorium turgor generation.
WebAug 4, 2014 · (A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying … WebJul 5, 2016 · The appressorium turgor was measured using an incipient cytorrhysis (cell collapse) assay and a 1–4 m glycerol solution (Howard et al., 1991). Droplets (20 µL) of the conidial suspensions (5 × 10 4 spores/mL) were placed on plastic coverslips and incubated in a humid chamber for 24 h at room temperature.
WebFeb 18, 2024 · Considering that the germinating and appressorial formation were normal in the Mohat1 mutant (Table S1), we measured the internal concentration of solute and the turgor pressure within appressoria by the incipient cytorrhysis (cell collapse) assay using 1–4 molar of glycerol [ 45 ].
WebApr 7, 2024 · Appressorium turgor pressure was analyzed by the incipient cytorrhysis assay using 1–4 M of glycerol solution, as described by Wang et al. (2024). The experiment was conducted three times with at least 100 structures per replicate. Onion penetration assays were conducted as described by Wang et al. (2024). the nztcWebJul 28, 2015 · The incipient-cytorrhysis method was used to compare the appressorial turgor. Treated with 0.5, 1 or 2 M glycerol, the appressoria of KO11A collapsed at … the nz vegetable gardenWebIn an incipient cytorrhysis (cell collapse) assay using a 1–4 molar concentration of glycerol solution, appressoria of the ΔMomip11 mutant showed increased collapse rates in … the nz rum coWebJan 26, 2024 · To investigate whether the reduced pathogenicity of ΔMopcs60 was associated with appressorium-mediated penetration, we first determined the rate of appressorium collapse in the presence of various hyperosmotic concentrations of glycerol by performing an incipient cytorrhysis assay. the nz wine companyWebApr 16, 2024 · The penetrating peg, with the aid of the mechanical force, ruptures the leaf cuticle and epidermal cell wall and differentiates subsequently into invasive hyphae that ramify within the host cell and spread from cell to cell, resulting in visible lesions and rice blast symptoms [ 2 ]. then 同义替换the nz storyWebNov 2, 2015 · Appressorium turgor was measured by incipient cytorrhysis assay using a 2- to 5-molar concentration of glycerol solution, as previously described (Foster et al ., 2003 ). Visualization of glycogen and lipid during appressorium development Appressorium formation was induced on the coverslips (Fisher Scientific). the nz treasury