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Fastpfu buffer

WebThe PCR mixtures contain 5 × TransStart FastPfu buffer 4 μL, 2.5 mM dNTPs 2 μL , forward primer (5 μM) 0.8 μL, reverse primer (5 μM) 0.8 μL, TransStart FastPfu DNA Polymerase … WebJan 30, 2024 · DNA samples were amplified in triplicate on an ABI GeneAmp ® 9700 PCR System (ABI, MA, USA) in PCR reactions containing 2 μl 2.5 mM dNTPs, 0.4 μl FastPfu Polymerase (Transgen Biotech, Beijing ...

Adaptation mechanisms of the soil microbial community under ...

WebConsequently, Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended. 10X Reaction Buffer with MgSO4: 200mM Tris-HCl (pH 8.8 at 25°C), 100mM KCl, 100mM (NH 4) 2 SO 4, 20mM MgSO 4, 1.0% Triton® X-100 and … Web本发明公开一种基于环境DNA技术监测淡水底栖动物群落多样性的方法，包括步骤：(1)采集表层水样；(2)水样过滤与eDNA提取；(3)PCR扩增；(4)高通量测序，获得OTU的代表序列；(5)底栖动物比对数据库的建立；(6)比对建立的底栖动物数据库，对待测样品OTUs代表性序列进行物种注释。本发明取样简单、人力 ... fenway park state street pavilion club https://sportssai.com

Characterization of microbiota of naturally fermented sauerkraut …

WebAug 11, 2024 · 吕燕 刘建利 李靖宇 候琳琳 孙敏 苟琪 （北方民族大学生物科学与工程学院，银川 750021） 宁夏枸杞（Lycium barbarum L.）为茄科，枸杞属，多年生落叶灌木，是典型药食同源性植物，是宁夏、甘肃、青海、内蒙古、新疆等少数民族地区脱贫的重要经济林品种，由于其极强耐旱、耐盐碱、耐贫瘠和抗沙荒 ... WebJul 12, 2024 · The PCR mixtures contain 5 × TransStart FastPfu buffer 4 μl, 2.5 mM dNTPs 2 μl, forward primer (5 μM) 0.8 μl, reverse primer (5 μM) 0.8 μl, TransStart FastPfu DNA Polymerase 0.4 μl, template DNA 10 ng, … WebApr 10, 2024 · PCR was performed in triplicate in a 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase, and 10 ng of template DNA. Amplicons were extracted from 2% agarose gels and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences ... delaware road closures

快速Pfu DNA聚合酶

Category:TransStart® FastPfu Fly DNA Polymerase ABIN5519533

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Fastpfu buffer

PEM-seq comprehensively quantifies DNA repair outcomes during …

WebFeb 8, 2016 · TransStart FastPfu Fly DNA Polymerase is a hotstart, ultra-high fidelity and high-processivity DNA Polymerase. It consists in a modified DNA Polymerase, … WebApr 28, 2024 · The raw materials used for PCR reaction were as follows: Transgen AP221–02:Transstart FastPFU DNA Polymerase, 20μL reaction system: 5 × TransStart FastPfu buffer (4μL), 2.5 mM dNTPs (2μL), forward primer (5μM) 0.8μL, reverse primer (5μM) 0.8μL, TransStart FastPfu polymerase 0.4μL,BSA 0.2μL, template DNA 10 ng, up …

Fastpfu buffer

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WebMar 18, 2024 · Cell lysis buffer Store it at room temperature (22°C–26°C, hereafter) for up to 6 months. CRITICAL: SDS is toxic. Wear gloves and a mask to avoid skin exposure and inhalation. TE buffer Store it at room temperature for up to 1 year. 1× B&W buffer Store it at room temperature for up to 1 year. 10 mM Tris-HCl, pH7.5 WebNov 29, 2024 · The PCR reaction volume usually uses 50μl system and consists of 5–30ng Plasmid DNA or 100ng Genomic DNA, 1x TransEco FastPfu Buffer, 250μM dNTP, 0.4μM of each primer, 2.5U of TransEco FastPfu DNA Polymerase or Taq DNA polymerase and ddH 2 O up to 50μl.

WebDec 23, 2024 · Each 20 μL reactions mixture, including 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of forward and reverse primers (5 μM), 0.4 μL of FastPfu Polymerase and 10 ng of template DNA were for PCR amplification in triplicate. WebApr 7, 2024 · Methods. Amplification, library preparation and sequencing of the bacterial 16S rRNA gene. The highly variable V3-V4 region of the 16S rRNA gene was amplified from …

WebWhat does buffer overflow actually mean? Find out inside PCMag's comprehensive tech and computer-related encyclopedia. #100BestBudgetBuys (Opens in a new tab) …

Web5x FastPfu FLY Buffer (with Mg2+) – GK221. $ 33.00 CAD. Format. Buy 1 x 1.2 ml 5x Fly Buffer for 33 $. Buy 5 x 1.2 ml 5x Fly Buffer for 120 $. Buy 25 x 1.2 ml 5x Fly Buffer for …

WebProduct Details. TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase. • Extension rate is about 2-4 kb/min. • … fenway park stadium tourshttp://school.freekaoyan.com/bj/wswyjs/2024/12-26/16405189191500959.shtml delaware roadWeb引物ITS3F（5'-GCATCGATGAAGAACGCAGC-3'）和ITS4R（5'-TCCTCCGCTTATTGATATGC-3'）：武汉天一辉远生物科技有限公司；QIAGEN DNeasy mericon Food Kit试剂盒：德国QIAGEN公司；5×FastPfu Buffer、2.5 mmol/L脱氧核糖核苷三磷酸（deoxy-ribonucleoside triphosphates，dNTPs）、FastPfu Polymerase、牛血 … delaware road clarence ny hauntedWebApr 11, 2024 · PCR reactions were performed in quadruplicate using a 20 μL mixture consisting of 4 μL of 5× TransStart FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of Forward Primer and Reverse Primer (5 μM), 0.4 μL of FastPfu Polymerase, 0.2 μL BSA, 10 ng Template DNA, and supplemented with sterile water to 20 μL. The qPCR product was … fenway park stadium seats for saleWebMar 30, 2024 · The PCR mixtures contain 4 μL of 5 × TransStart FastPfu buffer, 2.5 mM dNTPs 2 μL, forward primer (5 μM) 0.8 μL, reverse primer (5 μM) 0.8 μL, TransStart FastPfu DNA Polymerase 0.4 μL, template DNA 10 ng, and finally ddH2O up to 20 μL. The PCR reactions were carried out in triplicate. fenway park summer concert seriesWebCharacteristics. - TransStart® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase. - PCR products can be directly cloned into … delaware road conditions todayWebMar 8, 2024 · We then collected below-ground root biomass to a depth of 30 cm using soil cores (diameter 7 cm) in each of these five quadrats as well. Roots were collected by … delaware road closures todayelaware today